Wednesday, May 27, 2015

When Should I Use Flow Cytometry Isotype Controls?

The modern world is changing quickly, and nowhere is that more true than in the world of science. You may have used something last week or last month, only to find out it has become old and obsolete, and all of a sudden you're living in the past.


Antibody Isotype Controls | Expert Cytometry | Immunoglobulin Isotype Switching
The Importance Of Isotype Controls In Flow Cytometry
This is true in the field of flow cytometry as well. Take isotype controls, for example. At one point these weren't just the most effective negative controls, they were the only ones! And now, many believe their time is long gone.

But are these claims true? Is it time to put isotype controls out to pasture and move on?

Find out more on isotype controls (◄ read the full article here), and how you should (or shouldn’t!) make use of them in your tests from ExCyte, the leading authority on flow cytometry!

Of course, knowing when to use isotype controls is merely one of the numerous factors you need to know about flow cytometry clinical applications! One more is knowing when to use the right flow cytometry analysis software!!

Friday, May 15, 2015

Flow Cytometry In Clinical Diagnosis

Flow cytometry has made a big impact in the scientific world over the last several years, and its uses have gotten more common.

Clinical Flow Cytometry Experiments | Expert Cytometry | Applications Of Flow Cytometry
Running An Effective Flow Cytometry Experiment
So for those serious about boosting their STEM career, or getting it off to a good start, it doesn't get much better than this!

But clinical flow cytometry experiments are not an easy thing to do. Even the most skilled flow cytometrists could wind up missing out on certain crucial details and coming up with bogus or incorrect data.

Follow these Six tips, though, and your knowledge of and proficiency in flow cytometry clinical applications (◄ check out the full post here) will be better for it!

To get more on flow cytometry, like the significance of fluorescence staining and FACS data analysis, these posts will help!

Monday, April 27, 2015

The Best Way To Perform A SPICE Analysis With FlowJo

Flow cytometry is an ever-evolving field, and one element that's becoming exceptionally more complex is the collection of data. The good old days where just a couple colors were plenty enough to perform a valid experiment are long past. Now even the newest flow cytometrists use at least five colors, often more!

SPICE Data Analysis | Expert Cytometry | Flow Cytometry Analysis
The Right Way To Execute A SPICE Analysis With FlowJo

Naturally, the greater the test the more data there are to read, the more time it will take, and the more headaches you need to deal with!

So what can we do about this? If, like the majority of flow cytometrists, you’re using the flow cytometry analysis program FlowJo, you can incorporate the SPICE model into your experiments to make it much easier to read your data!

What is SPICE? It means Simplified Presentation of Incredible Complex Evaluations, and it’s vital for your flow cytometry data analysis! (< take a look at the full post here)

By the way, here are 10 FlowJo X tricks which will make flow cytometry analysis much less difficult!

And when you organize a flow cytometry experiment, do you know about fluorescence minus one controls? Discover more about them with this article!

Thursday, April 9, 2015

What Is FMO Control?



In the world of flow cytometry, there are all sorts of systems available to help you interpret your data and make your life a little easier. 
Fluorescence Minus One | Expert Cytometry | Fluorescence Assisted Cell Sorting

One of these tools is the Fluorescence Minus One control, or FMO control. And if you’re in the world of flow cytometry, you better know how it works! 
Read this latest article from ExCyte, the premier expert in flow cytometry, to discover more about Fluorescence Minus One ( read the full article here) controls and how they can help you!